Following its characterization as an activated oncogene (based on its ability to transform normal fibroblasts) Met was characterized as the receptor for hepatocyte growth factor.
The c-Met receptor is a disulfide bonded heterodimer consisting of an ~50kD extracellular a-chain and an ~145kD b-chain with a cytoplasmic tyrosine kinase domain.
Both chains are derived from a single precursor protein by proteolytic cleavage.
c-Met is expressed in a wide variety of adult and embryonic tissues.
It transmits biological responses including mitogenesis, morphogenesis and anti-apoptotic activity elicited by HGF.
Dysregulated c-Met gene expression is observed in a variety of human carcinomas and sarcomas.
c-Met also mediates the movement and invasiveness of neoplastic cells and promotes metastasis.
Recently, transcription of the c-Met gene was suggested to be a target for regulation by p53.
Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): Requires HIER using sodium citrate buffer pH6.0 followed by microwaving for 8-15min prior to staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins, no preservatives added. Glycerol free. Available with azide, See M3007-13.
Specificity：Recognizes the human c-Met protein at ~145kD. Does not crossreact with related proteins.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa located within last 50aa derived from the C-terminus of the human c-Met protein.
