84-1060-18　［取扱停止］STEAP (Six Transmembrane Epithelial Antigen of the Prostate) 100ug　S7500

特徴

• Prostate cancer is the most frequently diagnosed cancer and is the second leading cause of cancer-related deaths in American men.
• Although the early serum detection of prostate-specific antigen (PSA) plays important roles in early diagnosis, it may not distinguish prostate cancer from benign diseases.
• Recently, a novel protein, which contains 339 amino acids, named STEAP (six transmembrane epithelial antigen of the prostate), was identified in advanced prostate cancer.
• STEAP is unique among the currently known prostate cancer markers because of its putative secondary structure, from which one may predict that it functions as a potential channel protein.
• STEAP is strongly expressed in advanced prostate cancer and some extent expression in other cancers, such as colon and ovarian cancer cell lines.
• The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue (1,2).
• The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity (3).
• However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers.
• Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy (4).
• At least three isoforms of STEAP1 are known to exist.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-1.0ug/ml.
• Western Blot: 0.5-2.0ug/ml.
• By Western blot, this antibody detects a band of approximately 40kD in A431 cell lysate, which corresponds to the predicted molecular weight of STEAP.
• Sample was boiled prior to subject 10%SDS-polyacrylamide gel electrophoresis.
• Immunoprecipitation: 3-5ug/extract from 10e7cells.
• Optimal dilutions to be determined by the researcher.
• Positive Control: Human spleen tissue lysate
• Control Peptide: S7500P
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.