84-1060-16　PHD3 (Prolyl Hydroxylase Domain Containing Protein 3, HIF-Prolyl Hydroxylase 3, EGLN3) 100ul　P3375-05

特徴

• HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF-alpha.
• Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen.
• HIF is a transcriptional complex that plays a critical role in oxygen homeostasis.
• HPH is an essential component of the pathway through which cells sense oxygen.
• In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction.
• Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis.
• HPH-1, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins.
• It hydroxylates HIF-1 alpha at Pro(564) and HIF-2 alpha.
• It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.
• It may also play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissues, promoting cell death through a caspase-dependent mechanism.
• Applications:Suitable for use in Western Blot., Immunohistochemistry, Immunofluorescence/Immunocytochemistry, Immunoprecipitation and Electron Microscopy.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000-1:2000Immunofluorescence (IC): 1:500Immunohistochemistry (Paraffin): 1:250-1:1000Optimal dilution to be determined by the researcher.
• Positive Control:HeLa cell lysates
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.