Oxygen radicals damage chromosonal DNA causing cell death and inducing mutations.
Although a major focus of oxidatively damaged DNA has centered on the repair of 8-oxo-G, a number of other damaged DNA sites are created by free-radical attack on DNA.
The human NTH1 repair protein is one enzyme that has been shown to act on a large number of these other oxidatively damaged DNA sites (2).
A homologue of E.
coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity.
The NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines.
Research indicates NTH plays a crucial role in removal of oxidative base lesions in mitochondrial DNA (3).
Application(s):Suitable for use in Western Blot where a band at ~37kD is seen on SDS-PAGE.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1,000-1:5,000 (ECL)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
