84-1060-10　G-Protein Coupled Receptor 39 (GPCR39, GPR39) 50ul　G8600-43A

特徴

• GPR39 (G-protein coupled receptor 39) is an orphan GPCR receptor from ghrelin/motilin receptor subfamily of G-protein coupled receptor 1 family, and it is constitutively active with Zn2+ as its physiological agonist.
• GPR39 is a multipass transmembrane protein found in cell membranes and is expressed in several tissues such as stomach, intestine and hypothalamus.
• Obestatin, a 23 amino acid peptide originating from ghrelin precursor, was originally shown to be the endogenous ligand of GPR39, a study that happened to be non-reproducible, and later on it was shown that obestatin does not bind, nor activate GPR39 but that Zn2+ could stimulate GPR39 signalling in vitro, thereby inferring that obestatin is not the natural ligand of GPR39.
• GPR39 receptor also displays a strong ligand-independent signaling activity through G-alpha12/13 as well as G-alphaq.
• This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
• GPR39 has been shown to implicate in the regulation of insulin release/pancreatic function, body weight, gastrointestinal mobility, hormone secretion and cell death.
• Applications:Suitable for use in Immunocytochemistry/Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (paraffin): 1:50, prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.