GPR35 expression was reported only in rat intestine (O'Dowd et al., 1998) by Northern hybridization; however, another study showed that GPR35 is expressed widely, including pancreatic islets and skeletal muscle, with higher levels in adult lung, small intestine, colon, and stomach.
ESTs have been isolated from human fetal lung/testis/B-cell, colon (normal, cancer, and Crohn's disease), breast cancer, pancreatic cancer, and leukemia libraries.
Applications:Suitable for use in Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (formalin fixed paraffin embedded tissues): 5-9ug/ml Optimal dilutions to be determined by the researcher
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.7, 0.01% sodium azide, before the addition of glycerol to 40%.
Specificity:Recognizes human GPCR35. Species sequence homology: mouse 79%.
Isotype:IgG
Calc Applications Abbrev:IC IHC
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide made to the 3rd Cytoplasmic Loop (KLH coupled). Genbank accession number: NM_005301