84-1060-05　COP 1, alpha (Coatomer Protein 1 alpha) 100ug　C7900-45

特徴

• Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport.
• There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct.
• The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer.
• Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF).
• Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF.
• COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex.
• By contrast, COPII facilitates anterograde transport between these subcellular organelles.
• COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.
• Application:Suitable for use in Immunocytochemistry/Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry/Immunofluorescence: 3ug/ml, staining of alpha COP I using C7900-45 results in alpha COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm
• Western Blot: 1ug/ml, detects an ~140kD protein representing COPI alpha from PC-12 cell extract.
• Also detects an unidentified protein at ~100kD.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.