Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh).
Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it.
AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane.
AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission.
Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.
Applications:Suitable for Imminoblot (Western or Dot Blot), ELISA, Immunoprecipitation, Conjugation and most immunological methods.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000-1:5000; 1ug of Acetyl Cholinesterase was used in a standard sandwich ELISA with rabbit anti-goat (HRP)and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：bovine
Grade：Highly Purified
Purity：Purified by delipidation, salt fractionation and ion exchange chromatography.
Form：Supplied as lyophilized powder in PBS, 0.01% sodium azide. Reconstitute with 100ul sterile dH2O.
Specificity：Recognizes bovine Acetyl Cholinesterase. Assay by IEP resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Acetyl Cholinesterase.
