YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes.
It also binds to other SH3 domain containing proteins such as Nck, Crk, Src and Abl.
In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain and WW domains (2–4).
While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies show that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors.
YAP interacts with 14-3-3 in an Akt-dependent manner upon phosphorylation of Ser127 and suppresses p73-mediated apoptosis.
Applications:Suitable for use in Western Blot, ELISA and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4ºC with gentle shaking, overnight
Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Specificity：Recognizes endogenous levels of human total YAP protein at 65-75kD. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to the human sequence of YAP
