84-1059-92　PI 4-Kinase (Phosphatidylinositol 4-Kinase) 100ul　P4186-27

特徴

• Inositol phospholipids have an important role in intracellular signaling in response to hormones, growth factors and neurotransmitters.
• Phosphatidylinositol 4-kinase phosphorylates phosphatidylinositol (PI) to phosphatidylinositol-4-phosphate (PIP).
• In a second step, PIP is further phosphorylated to phosphatidylinositol-4,5-bisphosphate (PIP2), and PIP2 is subsequently hydrolyzed by phospholipase C, producing the two intracellular second messengers, inositol 1,4,5-triphosphae (IP3) and diacylglycerol (DAG).
• PI4K230, PI4K92 and PI4K55 are three PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92 and 55 kD.
• Previously, PI4 kinases were classified into type II and III enzymes.
• All isoforms are located on distinct membranes and cellular compartments suggesting various tasks.
• PI4K230 is located at the endoplasmatic reticulum and outer membranes of mitochondria, PI4K92 at the Golgi apparatus and endoplasmatic reticulum, and PI4K55 at the plasma membrane and endosomes.
• PI4K230 is predominantly expressed in brain and moderately sensitive to wortmannin as well as specifically and irreversibly inhibited by cyclitol derivatives.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:50Immunofluorescence (IC): 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.