Three distinct PCTAIRE isoforms (PCTAIRE 1, PCTAIRE 2 and PCTAIRE 3) have been identified in humans and belong to the Cdk family of serine/threonine protein kinases.
These proteins have a core kinase domain flanked by unique amino-and carboxy- terminal domains.
Cdk proteins are known to regulate the cell cycle.
All three PCTAIRE isoforms are abundantly expressed and catalytically active in post-mitotic brain, suggesting that they may function in processes other than cell division (1).
PCTAIRE 1 is a cytoplasmic phosphoprotein whose kinase activity peaks in G2 and S phase (2).
While one study indicates that noncovalent interactions with a regulatory component (such as a cyclin) are necessary for catalytic activity of PCTAIRE 1, others show that the monomeric protein is fully active (3).
The Cdk5/p25 complex phosphorylates PCTAIRE 1 at Ser95, enhancing its kinase activity (4).
Application:Suitable for use in Western Blot, Flow Cytometry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Flow Cytometry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
