84-1059-89　PBK, phosphorylated (Thr9) (PDZ Binding Kinase, Cancer/testis Antigen 84, CT84, Lymphokine-activated Killer T-cell-originated Protein Kinase, MAPKK-like Protein Kinase, Nori-3, Spermatogenesis-related Protein Kinase, SPK, T-LAK Cell-originated Protein Kinase, TOPK) 100ul　P3113-97E

特徴

• PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1).
• PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-Binding domain, which is thought to interact with the tumor suppressor protein hDlg (1).
• Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3).
• PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclinB has been shown to phosphorylate PBK/TOPK in vitro, presumably at Thr9 (1).
• Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4).
• Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunocytochemistry .
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Flow Cytometry: 1:25
• Immunocytochemistry: 1:100
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.