The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations.
Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1).
In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function (2).
While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors (2).
Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck (2,3).
The p63 gene contains an alternative transcription intiation site that yields a 40kD DNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the a, b and g isoforms (3,4).
TAp63-a (fulllength) contains multiple serine residues followed by proline (Ser-Pro motif) that are potential cdk substrates expected to be phosphorylated in mitosis.
Among these are Ser160, Ser162, Ser395, and Ser455.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human p63 only when phosphorylated at serine 160/162.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser160/160 of human TAp63-a. Species sequence homology: mouse, rat, chicken and xenopus
