The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations.
Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis.
In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function.
While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors.
Amplification of the p63 gene is frequently observed in squamous cell carcinomas of the lung, head and neck.
The p63 gene contains an alternative transcription intiation site, which yields a 40kD delta, Np63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the alpha, beta and gamma isoforms.
Applications:Suitable for use in Western Blot, Immunoflourescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoflourescence (IC): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, <0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human p63, alpha at ~75kD. Detects both full length TAp63, alpha and truncated delta, Np63, alpha, but not p63, beta or p63, gamma.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues within the C-terminus of human p63, alpha. Species Sequence Homology: chicken
