Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation, and apoptosis.
These proteins share a common basic-helix-loop-helix leucine zipper (bHLHZIP) motif required for dimerization and DNA-binding.
Max was originally discovered based on its ability to associate with c-Myc and found to be required for Myc’s ability to bind DNA and activate transcription.
Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families.
The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior.
The Mad family consists of four related protein designated Mad1, Mad2 (Mxi1), Mad3, and Mad4 and more distantly related members of the bHLH-ZIP family, Mnt and Mga.
Like Myc, the Mad proteins are tightly regulatedwith short half-lives.
In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation, and prevention of apoptosis by inhibiting trascription.
Applications:Suitable for use in Western Blot.
Other applications not tesetd.
Recommended Dilutions:Western Blot: 1:1000
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of Mad-1. Does not crossreact with other Mad family members at physiological levels. Species Crossreactivity: human, mouse, and rabbit
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminal region of Mad-1.
