The NFkB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins.
Most agents that activate NFkB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB.
The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits.
IKKa and IKKb serve as the catalytic subunits of the kinase.
IKKg serves as its regulatory subunit.
Activation of IKK depends on phosphorylation; serines 177 and 181 in the activation loop of IKKb (176 and 180 in IKKa) are the specific sites whose phosphorylation causes a conformational change that results in kinase activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human IKKb only when phosphorylated at serines 177/181 or 176/180. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Phospho-peptide corresponding to a region surrounding serines 177/181 of IKKb.
