The ubiquitin-conjugating (UBC) enzymes HR6A and HR6B are the mammalian orthologues of the Saccharomyces cerevisiae Rad6 gene products.
In S.
cerevisiae, Rad6 facilitates cell cycle progression and ubiquitinates histone H2B.
In vivo phosphorylation of HR6A Ser120 by cyclin-dependent kinases is thought to be important for the coordination and timing of ubiquitination events involved in cell cycle progression.
In response to DNA damage, HR6A is known to interact physically with p53 and p14ARF, but knockout mice lacking HR6A or HR6B exhibit normal DNA damage responses.
HR6B knockout males exhibit defective spermatogenesis, while HR6A knockout females fail to produce viable offspring.
Applications:Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot and Immunocytochemistry.
Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunofluorescence (IC): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Specificity：Recognizes endogenous levels of total human HR6A and HR6B proteins. Species Crossreactivity: mouse, rat, monkey.
Isotype：IgG
Calc Applications Abbrev：E FC IC IF WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids at the N-terminus of human HR6A. Species Sequence Homology: chicken, Xenopus and zebra fish, D. melanogaster.
