84-1059-81　Histone H2A 100ul　H5110-08M

特徴

• Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes.
• The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1).
• The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2–5).
• These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6).
• In most species, histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,7).
• Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3).
• Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3).
• Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8).
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunohistochemistry: (Paraffin) 1:200Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.