84-1059-78　Glycogen Synthase Kinase 3 alpha (GSK3a) 100ul　G8170-41C

特徴

• Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1).
• GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase.
• GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3a and Ser9 of GSK-3b (2,3).
• GSK-3 has been implicated in the regulation of cell fate in Dictyostelium, and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development (4).
• GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).
• GSK-3a regulates the production of amyloid-b peptides, a major component of the plaques that accumulate with progression of Alzheimer’s disease.
• Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-b production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by g secretase, blocking accumulation of amyloid-b peptides in the brains of mice that overproduce APP (6).
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.