The ezrin, radixin and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling and microvilli formation (1).
ERM proteins undergo intra-or intermolecular interaction between their amino-and carboxy-terminaldomains, existing as inactive, cytosolic monomers or dimers (2).
Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin), which disrupts their amino-and carboxy-terminal association, may play a key role in modulating the conformation and function of ERM proteins (3,4).
Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogeneinduced transformation (5).
Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation.
Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total ezrin protein. Does not crossreact with ezrin homologues such as radixin and moesin. Species Crossreactivity: human, mouse, rat, monkey and bovine
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu Mk Mo Rt
Immunogen：Synthetic peptide (KLH-coupled) corresponding to human ezrin.
