Phosphorylation of the a subunit of eukaryotic initiation factor 2 is a well documented mechanism of down regulating protein synthesis under a variety of stress conditions.
Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers the Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2).
For eIF2 to promote a new round of translation initiation, GDP must be exchanged for GTP, a reaction catalyzed by eIF2B (1,2).
Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the a subunit of eIF2 (3,4).
This phosphorylation stabilizes the eIF2-GDP-eIF2B complex, inhibiting the turnover of eIF2B.
Induction of PKR by IFN-g and TNF-a, or stress provoked by depletion of endoplasmic reticulum calcium levels, induces potent phosphorylation of eIF2a at Ser51 (5,6).
eIF2B, a guanine nucleotide exchange factor, is composed of 5 subunits, the largest of which is eIF2B-e (7).
Multiple in vivo phosphorylation sites have been identified on eIF2B-e (8).
Casein Kinase II can phosphorylate eIF2B-e at Ser717/718 to allow for association with its substrate eIF2.
Phosphorylation at Ser544 allows GSK-3 to phosphorylate the key regulatory site Ser540.
A fifth eIF2B-e phosphorylation site, Ser466, can be phosphorylated by casein kinase I.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes human endogenous levels of total eIF2B-e protein. Species Crossreactivity: Mouse, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids near the middle of human eIF2B-e (KLH). MW: 85kD.
