84-1059-74　CBP, acetylated (Lys1535)/p300 (Lys1499) (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS, Rubinstein-Taybi Syndrome) 100ul　C2098-10

特徴

• CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2).
• CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2).
• Phosphorylation of p300 at Ser89 by PKC represses its transciptional acitivity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4).
• Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPb (5).
• Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6).
• CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS(7).
• The role of acetylation of CBP/p300 is of particular interest (2,8).
• Acetylation of Lys1499 of p300 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).
• Applications:Suitable for use in Western Blot, Immunoprecipitation and ChIP.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50ChIP: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.