Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2).
Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47kDa)/Apaf 1.
This complex processes procaspase-9 into a large active fragment (35kDa or 17kD) and a small fragment (10kD) by self-cleavage at Asp315 (3–5).
Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7 to initiate a caspase cascade, which leads to apoptosis (6–9).
In addition to self-cleavage, procaspase-9 also can be cleaved in vivo by caspase-3 at Asp330.
This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway (3-5).
Applications:Suitable for use in Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry: (IF) 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of the 37kD subunit of mouse Caspase-9 only after cleavage at aspartic acid 353. Does not crossreact with full length caspase-9 or with other caspases at endogenous levels.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide (KLH-coupled) corresponding to residues surroundingAsp353 of mouse caspase-9.
