84-1059-72　APS 100ul　A2500

特徴

• APS is an SH2 and PH domain-containing adaptor protein closely related to Lnk and SH2-B (1).
• APS was identified as a substrate for many receptor tyrosine kinases including TrkA, insulin receptor, c-Kit and PDGF receptor (2).
• Tyrosine phosphorylation of APS provides docking sites for downstream signalingcomponents, mediating diverse signaling pathways.
• APS plays quite different roles in RTK signaling.
• Overexpression of APS has been shown to inhibit PDGF-induced mitogenicity, which may result from APS/c-Cbl-mediated PDGF receptor degradation (3).
• However, APS promotes enhanced mitogenicity in response to insulin stimulation (4).
• The striking difference in APS-mediated signaling between the different RTKs could lie in the mode of interaction with the respective receptor.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.