CD262, otherwise known as DR5/TRAIL-R2, is a broadly expressed death domain containing receptor and member of the TNF-receptor superfamily, which acts as a mediator for CD253/TRAIL induced apoptosis in a variety of tumor and transformed cells, but not in normal cells.
Expression of CD262 is regulated by tumor suppressor p53, and CD262 is highly expressed by tumor cell lines.
Studies suggest that the death domain containing adaptor protein FADD, is required for CD262-mediated apoptosis, and that FADD recruits caspase-8 to the activated receptor.
Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA (indirect): 1:75,000Western Blot: 1:500Immunohistochemistry (paraffin): 1:150Flow Cytometry: 1ul (~10e6 cells/100ul)Optimal dilutions to be determined by the researcher.
Positive Control:Spleen
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Specificity：Recognizes human TRAIL-R2. Crossreacts <0.2% with TRAIL-R3 peptide of the same region; not likely to bind TRAIL-R1 due to low sequence homology in this region. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the extracellular domain sequence, aa68-94, APQQKRSSPSEGLCPPGHHISEDGRDC, of human TRAIL-R2.
