84-1059-67　［取扱停止］Dok2, p56 (Docking Protein, Downstream of Tyrosine Kinase, Dok-R, p56Dok2, FRIP, rasGAP-associated docking protein) 100ug　D8080-02C

特徴

• The binding protein Dok-2 is also known as p56dok-2.
• It is a 56kD tyrosine phosphorylated protein, purified and cloned from p210bcr-abl expressing cells, is able to bind to p120RasGAP.
• There is 35% identity to Dok-1 (p62dok-1).
• Dok-2 is a rasGAP-associated docking protein expressed mostly in hematopoietic cells.
• Dok-2 is phosphorylated upon activation of many protein tyrosine kinases (PTKs), including those coupled with cytokine receptors and oncogenic PTKs like Bcr-Abl.
• Loss of Dok-1 and/or Dok-2 in a knock out mice model carrying the bcr-abl gene resulted in progression of a myeloproliferative disease resembling human chronic myelogenous leukemia.
• The mouse model suggests that both Dok-1 and Dok-2 suppress leukemia as negative regulators of cytokines responses as well as maintain myeloid homeostasis.
• Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000.
• Detects ~60kD band
• Immunocytochemistry:1:300ELISA: 1:100,000 (peptide).
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.