In diploid eucaryotic cells, the chromatin fibers are about 20nM in diameter.
They consist of two major components in equal amounts, DNA and the basic proteins call Histones.
The recurring positive charges of the histones form electrostataic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible.
This allows for the supercoiling of the chromatin fibers.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:300Immunohistochemistry (paraffin): 1:100Immunoprecipitation: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：250ug
Host：sheep
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity：Recognizes bovine Histone H3.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo
Immunogen：Intact calf H-III complex to rRNA. Species sequence homology: 100% identity to many other mammalian histone H3 proteins.
