The EGFr which tranduces the biological effect of EGF is a 170kD glycoprotein containing two functional domains linked by a transmembrane region.
The extracellular domain is glyosylated possessing a single binding site.
The intracellular domain contains a protein tyrosine kinase.
Binding of EGF to receptor stimulates tyrosine kinase activity, essential for propagation of signal transduction.
Upon ligand binding, EGFr becomes autophosphorylated and also phosphorylates several other endogenous proteins including phospholipase cg.
These events initiate a number of intracellular responses which include increased levels of intracellular Ca2+ and transient expression of the nuclear oncogene products c-myc and c-fos.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 10-50ug/mlImmunohistochemistry (paraffin): 5ug/mlImmunoprecipitation: 10-12ug/mlImmunocytochemistry: Formalin-fixed cellsOptimal dilution determined by the researcher.
Positive Control:A431 epidermoid carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added.
Specificity：Recognizes the internal domain of the human Epidermal Growth Factor Receptor and will block the phosphoylaiton but not EGF. Recognizes mouse, rat, chicken receptors in Western Blots and in formalin fixed cell Immunocytochemistry.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Ch Hu Mo Rt
Immunogen：Synthetic 20 amino acid peptide corresponding to human EGFr representing a portion of the internal domain proximal to a phosphorylation region.
