Acrp30 (adipocyte complement-related protein of 30kD).
It is also known as AdipoQ, APM1, Adiponectin, Gelatin binding protein 28kD/GBP28 or adipocyte most abundant gene transcript.
It was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q.
Like adipsin, Acrp30 secretion is induced ~10-fold during adipocyte differentiation.
Plasma levels are reduced in obese humans.
Low levels are associated with insulin-resistance.
Treatment of db/db mice with TZD increased Acrp30 levels.
Acrp30 (mouse 247aa, rat human 244aa; chromosome 3q27) consists of a predicted NT-signal sequence 91-14aa), followed by a 27aa unique region, and then by 22 perfect Gly-X-Pro or Gly-X-X collagen like repeats, and a globular segment at the C-terminus.
Structurally, but at the sequence level, Acrp30 resembles other collagen-like and globular domain proteins (lung surfactant protein and hepatocytes mannan-binding proteins).
Acrp30 is proteolytically cleaved at 104aa to generate the globular Acrp30 (gAcrp30).
Administration of gAcrp30 into mice fed a diet high in fat and sugar caused substantial weight loss.
A marked reduction in plasma triglycerides, glucose, and free fatty acids was attributed due in part to increased fatty acid oxidation by muscle.
Full length Acrp30 was less potent than gAcrp30.
Therefore, gAcrp30 may open new avenues to control obesity.
Applications:Suitable for use in ELISA, Western Blot.
