L-type calcium channels are composed of five subunits.
The protein encoded by this gene represents one of these subunits, gamma, and is one of several gamma subunit proteins.
It is an integral membrane protein that is thought to stabilize the calcium channel in an inactive (closed) state.
This protein is similar to the mouse stargazin protein, mutations in which have been associated with absence seizures, also known as petit-mal or spike-wave seizures.
This gene is a member of the neuronal calcium channel gamma subunit gene subfamily of the PMP-22/EMP/MP20 family.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2.0ug/ml detects Stargazin in RIPA lysates from rat brain microsomal preparation.
Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Stargazin (0.5ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Immunoprecipitation: 2-5ug has been reported by an independent laboratory to immunoprecipitate Stargazin in rat P2 lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes rat Stargazin.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Rt
Immunogen：KLH-conjugated, synthetic peptide corresponding to amino acids 305-323 (KDSKDSLHANTANRRTTPV) of rat Stargazin at C-terminal. Species sequence homology: mouse
