Recommended Dilution:Western Blot: 1:1000 detects recombinant MKK7 1 that had been phosphorylated by MEKK1.
Recombinant MKK7 1 was left untreated or phosphorylated by MEKK1 and resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity：Recognizes MKK7/SKK4 when phosphorylated at Thr275 and Ser277, Mr 47kD. Species Crossreactivity: Human. Crossreactivity with mouse expected due to sequence homology.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acids surrounding Thr275/Ser277 of human MKK7/SKK4 (KLH).
