Applications:Suitable for use in Peptide Inhibition, Peptide Dot Blot, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Peptide Inhibition: 10uM of a histone H4 peptide containing acetyl-lysine 12 abolished detection of histone H4 by anti-acetyl-Histone H4 (Lys12) in immunoblot analysis of HeLa acid extracts.
Little to no signal reduction was observed with 10uM of histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16.
Peptide Dot Blot: A 1:1,000 detects serial dilutions (25, 5, 1 and 0.2uM) of a histone H4 peptide containing acetyl-lysine 12 spotted onto PVDF, and not histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16.
Western Blot: 1:3,000 to 1:10,000 detects acetyl-Histone H4 (Lys12) in acid extracted proteins from HeLa cells.
The antibody did not detect unacetylated recombinant Histone H4.
Immunoprecipitation: 2-6ul immunoprecipitated the Ime2 promoter from wild type yeast chromatin and not from mutant yeast chromatin containing a histone H4 K12R mutation.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum depleted of non-acetyl reactive antibodies.
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes histone H4, Mr 10kD. Species Crossreactivity: Yeast and human. Broad species crossreactivity expected.
Isotype：IgG
Calc Applications Abbrev：DB IP WB
Calc Crossreactivity：Hu Ye
Immunogen：Ovalbumin-conjugated, synthetic peptide containing the sequence (...LG[acK]GG...) in which acK corresponds to acetyl-lysine 12 of yeastHistone H4.
