Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eurokaryotic cells.
Featuring a main globuular domaina and a long N-terminal tail.
H3 is involved with the structure of the nucleosomes of the “beads of string” structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes.
These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Applications:Suitable for use in Western Blot, Immunofluorescence, Beadlyte Histone Peptide Assay and Peptide Dot Blot Analysis.
Recombinant histone H3 and chicken core histones were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-11N.
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Beadlyte Histone Peptide Assay: 1:10,000-1:80,000 detected only the histone H3 peptide containing the monomethyl lysine 36 modificationPeptide Dot Blot Analysis: 1:5000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification.
A dilution series of Histone H3 peptides.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes human monomethyl-Histone H3 (Lys36), Mr ~17kD. A non-specific protein was also detected, Mr ~200kD. Broad species crossreactivity is expected.
Isotype：IgG
Calc Applications Abbrev：DB IF WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide containing the sequence …GVmeKKP… in which meK corresponds to monomethyl Lysine 36 of human histone H3, conjugated to KLH.
