84-1059-06　TLR7 (Toll Like Receptor 7, TLR-7, PRO285, UNQ248) 100ug　T8050-73

特徴

• The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain.
• Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans.
• The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2).
• TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway.
• Ten human homologs of TLRs (TLR1-10) have been described (3).
• Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response.
• Applications:Suitable for use in Immunohistochemistry, Flow Cytometry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 2-5ug/1x10e6 (Intracellular staining)
• Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Ramos or Raw cells
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.