84-1059-01　［取扱停止］TLR2 (Toll Like Receptor 2) 100ug　T8050-14

特徴

• The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain.
• Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1).
• The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2).
• Ten human homologs of TLRs (TLR1-10) have been described (3).
• TLR2 is differentially expressed in human cells.
• TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers.
• CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2.
• The expression of TLR2 in different cell types is regulated by different immune response modifiers.
• For example, LPS, GM-CSF, IL-1, and IL-10 up-regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/ml.
• Detects a band at ~86kD
• Optimal dilutions to be determined by the researcher.
• Positive Controls:Whole Raw or Ramos cell lysate or mouse TLR2 transfected cell lysate.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.