The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain.
Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1).
The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2).
Ten human homologs of TLRs (TLR1-10) have been described (3).
TLR2 is differentially expressed in human cells.
TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers.
CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2.
The expression of TLR2 in different cell types is regulated by different immune response modifiers.
For example, LPS, GM-CSF, IL-1, and IL-10 up-regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlmouse TLR2 transfected cell lysate can be used as positive controlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes mouse TLR2 (Toll Like Receptor 2)
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：portion of the extracellular region of mouse TLR2.
