84-1058-94　TACI (Transmembrane Activator and CAML Interactor) 100ug　T0294-05

特徴

• Simultaneously four different laboratories identified a a new member of the tumor necrosis factor (TNF) family.
• This has been named as TALL-1 (1), THANK (TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase (2), BAFF (for B cell activating factor belonging to the TNF family) (3) and BlyS (B lymphocyte stimulator) (4).
• Membrane-bound BAFF is processed and secreted through the action of a protease whose specificity matches that of the furin family of proprotein convertases.
• The receptor for BlyS/BAFF appears to be expressed predominantly on B cells and activated T cells (5).
• Recently two orphan receptors TACI (6) and BCMA (7,8) have been shown to be receptors for BlyS/BAFF (6-9).
• TACI is expressed on B cells and signals through CAML, activating the transcription factors NF-AT, NF-kB, and AP-1.
• TACI is a type III transmembrane protein, as defined by the lack of a cleaved signal sequence and its N-terminal extracellular exposure.
• The cDNA codes for 293 amino acids with predicted molecular weight of approximately 32 kD.
• Applications:Suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml of TACI in 10ug/lane of Daudi lysate.
• A protein band of ~32 kD is detected.
• Optimal dilutions to be determined by the researcher.
• Positive Control:Human spleen cells, Raw cells, or Daudi cells
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.