The suppressor of cytokine signaling (SOCS) family of proteins (SOCS1-SOCS7 and CIS) inhibit cytokine production by a classical negative feedback mechanism.
These proteins are upregulated in response to cytokine stimulation followed by inhibition of cytokine-inducing signal pathway.
Role of each members of the family has been determined by gene knockout experiments (1).
SOCS1 also known as JAB and (STAT)-induced STAT inhibitor 1 (SSI-1) is an SH2-domain-containing protein that is induced by and negatively regulates signaling by a number of cytokines including interleukin-4 (IL-4), IL-6, interferon (IFN)-gamma, prolactin, growth hormone, and erythropoietin (1).
SOCS1/JAB is rapidly induced by LPS and negatively regulates LPS signaling (2).
SOCS1 (-/-) mice are very sensitive to LPS.
These mice die prematurely, probably due to uncontrolled IFN-g signaling (3).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
positive control:Human Ramos or mouse Raw cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G chromatography
Form：Supplied as a liquid in PBS, 0.1mg/ml BSA, 0.02% sodium azide.
Specificity：Recognizes human SOCS-1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa54-68 (HFRTFRSHADYRRIT) of human SOCS-1 (suppressor of cytokine signalling-1). Species sequence homology: rat
