SMADs are members of the MAD-related family of molecules.
MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1).
SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 5, 8 and 9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7).
SMAD1, 5, 8 and 9 have high degrees of homology and antibodies are available that recognize sequences common to all of them.
SMAD8 and SMAD9 are typically used as alternate names for one another in the literature.
For additional information on SMAD proteins, please follow the provided links to the NCBI website: human SMAD1, SMAD2, SMAD3, SMAD4, SMAD5, SMAD6, SMAD7, SMAD9 and mouse SMAD8.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher.
Positive control: A549 whole cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography
Form：Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity：Recognizes human SMAD 1/5/8/9. Species Crossreactivity: mouse and rat.. May cross-react with SMAD 2 and 3.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptides corresponding to aa18-32, LGWKQGDEEEKWAEK, and aa315-329, SNVNRNSTIENTRRH of human SMAD1. Species Sequence Homology: chicken, chimpanzee, invertebrates, mammals, porcine, Xenopus and zebrafish.
