Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity.
Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively.
Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention.
SUMO, or Sentrin, is one of the most intriguing.
SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-3ug/ml observed size: 15 kD positive control: HL60 or NIH3T3 whole cell lysate Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes.Applications: Suitable for use in Western Blot. Other applications not tested.Recommended Dilution:Western Blot: 1-3ug/ml observed size: 15 kD positive control: HL60 or NIH3T3 whole cell lysate Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
