Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription.
PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of omega-N(G)-monomethylarginine and symmetric omega-N(G),N(G')-dimethylarginine in a variety of proteins.
Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein.
PRMT5 appears to have lower specific enzyme activity than PRMT1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1,000HeLa cell lysate can be used as positive control
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 200ul of PBS containing 0.02% sodium azide and 0.1 mg/ml BSA.
Specificity：Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of omega-N(G)-monomethylarginine and symmetric omega-N(G),N(G')-dimethylarginine in a variety of proteins. Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein. PRMT5 appears to have lower specific enzyme activity than PRMT1.Applications: Suitable for use in Western Blot. Other applications not tested.Recommended Dilution:Western Blot: 1:500-1,000HeLa cell lysate can be used as positive control.Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 24 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptides corresponding to human PRMT5 amino acid sequence.
