84-1058-66　PIAS, gamma (Protein Inhibitors of Activated STAT) 100ug　P4085

特徴

• The interaction of a cytokine with its specific cell surface receptor triggers the activation of intracellular signaling pathways that ultimately program the cellular response.
• The signal transducers and activators of transcription (STAT) proteins play important roles in the activation of these pathways.
• Recently, a family of proteins has been identified that inhibits activated STATS and are named as Protein Inhibitors of Activated STAT (PIAS).
• PIAS proteins comprise a family of five mammalian proteins, which have been identified as potentially important down regulators of the STAT-signaling pathway.
• The cDNA for mouse and human PIASg encodes a protein of 507 amino acids that is highly homologous to the human protein and is expressed in the mouse as early as d 7.5 of gestation.
• In situ hybridizations of staged mouse embryos localized the transcript for the PIASgamma gene to the limbs, neuroepithelium, and the inner root sheath of the hair follicle, suggesting a role in the development of these structures.
• This protein is also present in hair follicle of human scalp hair and in monkey neuronal cells.
• Thus PIASg exhibits a highly selective pattern of expression, suggesting that it modulates the response of cells to developmental cues.
• Applications:Suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1-2ug/ml.
• Optimal dilutions to be determined by the researcher.
• Positive control:Jurkat cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.