One of the major signaling pathways used by eukaryotic cells to translate extracellular signals into cellular responses is the MAP (mitogen-activated protein) kinase cascade.
So far, four MAPK subgroups (ERK, ERK5, JNK and p38) have been identified in humans.
ERK, extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases.
Applications:Suitable for the detection of ERK1 protein in western blot.
A431 whole cell lysate can be used as a positive control.
Western blot analysis of ERK1 in human A431 cell lysate using antibody at 1ug/ml.
Recommended Dilution:Western blot: 1-3ug/ml
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity：Recognizes human ERK1 (MAPK1)
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a portion of human ERK1 between amino acids 350-400.
