84-1058-45　Histone Deacetylase 4 (HDAC 4) 100ug　H5109-49A

特徴

• Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the eta-amino groups of lysines located near the amino termini of core histone proteins (1).
• Eight members of HDAC family have been identified in the past several years (2,3).
• These HDAC family members are divided into two classes, I and II.
• Class I of the HDAC family comprises four members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domain exhibiting from 45 to 93% identity in amino acid sequence.
• Class II of the HDAC family comprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofold larger than those of the class I members, and the deacetylase domains are present within the C-terminal regions, except that HDAC-6 contains two copies of the domain, one within each of the N-terminal and C-terminal regions.
• Human HDAC-1, 2 and 3 were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showed tissue-specific expression patterns (3).
• These results suggested that each member of the HDAC family exhibits a different, individual substrate specificity and function in vivo.
• Applications:Suitable for the detection of HDAC4 in mouse and human cells by Western blot at.
• A protein of 140kD is detected in Jurkat and NIH3T3 cells.
• Western blot analysis of HDAC4 in Jurkat cell lysate with antibody.
• Recommended Dilution:Western blot: 0.5-2ug/ml
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.