Apoptosis or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms.
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas.
A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5.
The ligand for this novel cell death receptor has not yet been defined.
DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen.
Like TNFR1, DR3 induces apoptosis and NF-kB activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500 to 1:1000Whole cell lysate from Jurkat cells can be used as positive control and a 59kD band is detected.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Recognizes human DR3, Wsl-1, Apo-3, TRAMP, LARD1-5 (Death Receptor3)
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to amino acid 398 to 417 at C-terminus of human DR3(1,2).
