Five distinct DNA ligase activities, I-V, have been identified in mammalian cell extracts.
One of these enzymes, LIG1 is central to DNA replication.
During DNA replication, the joining of Okazaki fragments by the LIG1 gene product appears to be mediated by an interaction with proliferating cell nuclear antigen (PCNA).
LIG1 participates in nucleotide excision and base excision repair.
Applications:Western blot analysis of Lig-1 in cell lysate from HeLa cells using antibody at 1:1000 dilution.
Recommended Dilution:Western blot: 1:500-1:1000
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 200ul of PBS containing 0.02% sodium azide and 0.1 mg/ml BSA.
Specificity：Recognizes human DNA ligase 1 (Lig 1)
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide containing amino acids 51-68 of human Lig-1.
