Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection.
Chemokines activate leukocytes by binding to G protein coupled receptors (1).
The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines.
One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ), eotaxin, as well as several other related chemokines (2-4).
Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).
Applications:Suitable for detection of CCR1 expression by Western blotting.
A band of approx.
size 42 kD should be detected.
Western blot analysis of 10ug of total cell lysate from A357 cells with antibody at 2 g/ml dilution.
Recommended Dilution:Western blot: 1-3ug/ml
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity：Cross reacts with mouse CCR1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide of human CCR1 protein.
