84-1058-26　BAFF (BlyS, THANK, TALL 1) 100ug　B0003-69

特徴

• Simultaneously four different laboratories identified a new member of the tumor necrosis factor (TNF) family.
• This has been named as TALL-1 (1), THANK (TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase (2), BAFF (for B cell activating factor belonging to the TNF family) (3) and Blys (B lymphocyte stimulator) (4).
• Membrane-bound BAFF is processed and secreted through the action of a protease whose specificity matches that of the furin family of proprotein convertases.
• Secreted Blys/BAFF/THANK acts as a potent B cell growth factor.
• This protein is expressed abundantly in peripheral blood lymphocytes, specifically in monocytes and macrophages.
• Its regulation is upregulated by interferon gamma.
• Overexpression of Blys/BAFF in transgenic mice lead to increased numbers of mature B and effector T cells.
• These mice also develop autoimmune –like symptoms, such as, high levels of rheumatoid factors, anti-DNA autoantibodies, etc.
• (5) Recently, two receptors for Blys/BAFF have been identified and termed as BCMA and TACI (6).
• Applications:Suitable for the detection of BLyS/BAFF expression in mouse and human cells by Western blot.
• Western blot analysis of BAFF/Blys in mouse and human cell lysates.
• Lane A: mouse testes, Lane B: mouse thymus, Lane C: mouse spleen, and Lane D: HL-60 cells (human).
• Recommended Dilution:Western blot: 0.5-2ug/ml
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
• Freeze at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.