Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis.
All three of the proteins must be present for toxicity to occur.
It begins with the binding of PA to its cell-surface receptor, ATR (Anthrax Toxin Receptor), at which point PA is cleaved and the resulting 63kD carboxyl-terminal fragment binds to LF and EF.
LF is a protease that is known to cleave members of the mitogen-activated protein kinase kinase (MAPKK) family (2).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Recommended Positve Control:Recombinant protein
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
