Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphtidylinositol 3-kinase (PI 3-kinase).
The major activity of Akt/PKB is to mediate cell survival.
Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity.
There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma).
Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein.
Akt/PKB phosphorylation is critical for its activity.
Applications:This antibody can be used for detection of AKT3 in human, mouse and rat by western blot analysis.
A protein band of approximate molecular weight of 50kD will be detected.
Recommended Dilution:Western blot: 1-2ug/ml
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide, 0.1mg/ml BSA.
Specificity：This sequence is identical in human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acid residues 119-136 of human Akt3 (6).
